| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,772 | 0 | A | G | 57.9% | 8.1 / 16.4 | 19 | G393G (GGA→GGG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base G (11/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCATTCTGGTTCTGGAGATGACC > NZ_CP009273/172684‑172841 | gTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGaa < 2:279465/90‑1 (MQ=255) aCTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATg < 2:224397/90‑1 (MQ=255) cTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTGTTTTGCCGGGATgg > 1:90856/1‑90 (MQ=255) gtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTTTTGCCGGGATtggg > 1:246415/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGGGTTTTGTTTTGCCCGGATGGGGGGAtt > 1:82330/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTGCCGGAATAGGGGTATTACTgg > 1:176367/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGAGTTTGCTTTTGTCGGGAAGGGGGGATTaaccg > 2:242406/1‑86 (MQ=255) gCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTggcg > 2:102789/1‑90 (MQ=255) tGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTTCCGGGATGGGGGGATTACTGGCGGCCTCTATTc > 1:499914/1‑90 (MQ=255) gCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGGGTTTTCTTTTTCCGGGATGGGGGGATTACTGGCGGCGTCTTTTtccgcg > 1:360481/1‑90 (MQ=255) cGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTTCCGGGATGGGGGGATTAATGGCGGCATCTATTTCCGCG‑cc > 1:388737/1‑90 (MQ=255) gAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTTTTTCCGGGATGGGGGGATTACTTGCGGCATCTATTCGCGCG‑CCGttt > 1:436760/1‑89 (MQ=255) gCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTGCCGGGATGGGGGCATTACTTGCGGCATCTATTTGCGCG‑CCGTTTAc > 1:171073/1‑90 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGCGTTTGTTTTTTCCGGGGTGGGGGGGTTTCTTGCGGGCTCTATTTGCGCGCCCGTTAAGgggg > 1:235934/1‑90 (MQ=255) atatCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCAttct < 2:176367/90‑1 (MQ=255) atatCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCAttct < 1:224786/90‑1 (MQ=255) atatCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCAttct < 2:499914/90‑1 (MQ=255) atatCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCAttct < 2:86685/90‑1 (MQ=255) gAGGCGGGGGCGTTTGCTATTGCCGGGATGGGGGGATTTTTTGCGGCATCTATTTGCGCG‑CCGGTAACGGGGATCATTCTTGGTCTGgag > 2:341094/1‑90 (MQ=255) gggACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCATTCTGGTTCTGGAGATGAcc < 1:421324/90‑1 (MQ=255) | GTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCG‑CCGTTAACGGGGATCATTCTGGTTCTGGAGATGACC > NZ_CP009273/172684‑172841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |