| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 58.8% | ‑2.5 / 16.5 | 17 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base A (0/10); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTA > NZ_CP009273/353515‑353683 | tttCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa > 2:412438/1‑90 (MQ=255) ccAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc > 1:118940/1‑90 (MQ=255) aacaacCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt > 1:458306/1‑90 (MQ=255) cacccGCGCTTTTACCCACCCCCTTGCAAAAAAATCCCTCCGGGGCGGCTGCCCCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 2:398654/87‑1 (MQ=255) cacccGCGCTTTTACCCAACGCCATGTGCAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTtc < 1:114954/87‑1 (MQ=21) cATTTACCCAACCCCATGTGAAAAAATGCCTTCGGGGGGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagt < 1:124226/90‑1 (MQ=25) tCGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGcc < 1:103479/90‑1 (MQ=255) ccggggcggtTGTCTGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGcc < 2:456715/86‑1 (MQ=255) ccggggcggcTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGcc < 1:412438/86‑1 (MQ=255) cggcggCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAAGAAAAAACTTAAACCCCg > 1:314562/1‑90 (MQ=255) gcTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCAtgt < 2:348082/90‑1 (MQ=255) tttttcCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:314562/89‑1 (MQ=255) tttttcCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:216838/89‑1 (MQ=255) tttttcCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGAAAG‑AGAAATAGCTCTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:454840/89‑1 (MQ=255) tttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 1:254569/90‑1 (MQ=255) gccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAG‑AGAAAAAAATTTTAACCCCCCCTGTTGTTTGGGGAACGCTGCTgggg > 1:185511/1‑88 (MQ=255) gccgaAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:387067/90‑1 (MQ=255) | TTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTA > NZ_CP009273/353515‑353683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |