Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273636,3360TG54.5% 6.9 / 11.9 11G376G (GGT→GGG) ahpFalkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGC  >  NZ_CP009273/636269‑636411
                                                                   |                                                                           
aaGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTGAGCACGTAACgctgc                                                       >  1:30415/1‑90 (MQ=255)
     aaaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATTGTTGGGCACGGAACGCTGCTggga                                                  >  1:288405/1‑88 (MQ=255)
     aaaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTGAGGACGGAACGCTGCTggga                                                  >  2:184597/1‑88 (MQ=255)
                           gcggTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTTAGCACGGAAACGTGCTGGAATTTGCGCCAAAAAAGaaaagcg                            >  1:418036/1‑86 (MQ=255)
                                  cTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGt                     <  1:107738/90‑1 (MQ=255)
                                  cTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGt                     <  1:198494/90‑1 (MQ=255)
                                     cGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGGTCt                  >  2:168450/1‑90 (MQ=255)
                                      ggCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTg                 <  2:172902/90‑1 (MQ=255)
                                                cGGCAATTGACCTGGCGGGGATCGTTGAGCACGTAAGGCTGCTGGGATTTTGGCCAGAAATGGAAGCCGCCCCAGGTCTGCAGGACAAAc       >  2:301793/1‑90 (MQ=255)
                                                     aTTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTgcgc  <  2:143999/90‑1 (MQ=255)
                                                     aTTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTgcgc  <  2:189663/90‑1 (MQ=255)
                                                                   |                                                                           
AAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGC  >  NZ_CP009273/636269‑636411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑