| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,295 | 0 | A | C | 52.9% | 17.8 / 22.2 | 17 | F15V (TTT→GTT) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base C (0/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGT > NZ_CP009273/1500221‑1500384 | ggtggCCCGGGCAAAAATCGCTGCTTGCAAGATTTTTGCCGCGGAACGGGGGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCgt < 2:111280/90‑1 (MQ=255) ggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtggg > 2:55217/1‑90 (MQ=255) ggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtggg > 2:442661/1‑90 (MQ=255) gccccGGCGACAACCGCTGCTTGCCAGATTATTGCCGCTGAACGGGCGTAGCCAATTAATCCCGCCAAAACCCCCGCCAGTAGCgtgggt < 1:326456/87‑1 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 2:85363/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:274959/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 2:23099/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:104337/1‑90 (MQ=255) ggCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTggagg < 1:55217/90‑1 (MQ=255) ggCGACAACCGCTGCTTGCAAGATTATTGCCGCTAACCGGGCGTAGCCAATTAATCCCGCAAGAACCCCCGCCAGTAGCGTGGGTggagg < 1:442661/90‑1 (MQ=255) tttGCAAGATTATTGCCGCTAAACTGGCGTAGCAAATTAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 1:230458/89‑1 (MQ=255) ctTGCCAAATTATTGCCGCTAAATGGGCGTAGCAAATTAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 1:276246/90‑1 (MQ=255) ttGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCt > 1:312583/1‑90 (MQ=255) gCCGCTAACCGGGGGTAACAAATTAACCCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg < 1:290961/90‑1 (MQ=255) gCCGCAAAACGGGCGAACCAAATAAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg < 2:104337/90‑1 (MQ=255) agagcTGGCATAGCCAATCATCCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGt < 1:23099/86‑1 (MQ=255) gCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAtt > 2:56900/1‑90 (MQ=255) aCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGt < 1:56900/90‑1 (MQ=255) | GGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGT > NZ_CP009273/1500221‑1500384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |