| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,152 | 0 | C | A | 60.0% | 1.4 / 12.2 | 10 | A321S (GCC→TCC) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base A (0/6); total (3/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAA‑CAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCA > NZ_CP009273/2356073‑2356238 | gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAA‑CAGCCAGTGCCAGCCCGGCAAAATGGTTg > 2:448997/1‑90 (MQ=255) ccGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAA‑CAGCCAGTGCCAGCCCGGCAAAATGGTTGGCACGCCGt < 1:237217/90‑1 (MQ=255) ccGTCGGGGGTGGCTAGCGAAAAGTTGAGATGCTCTTTCTCGAAACCCA‑AAA‑CAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGt < 1:448997/90‑1 (MQ=255) gttgggCGTGGCTAGCGAAAAGTGGAGATGCTCGTTCCCAAACCCCG‑AAA‑CAGCCAGTGCCAGCCGGGAAAAATGGTTGGCCCGCCGTTc < 2:260420/86‑1 (MQ=255) ggCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAA‑CAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCa > 1:4946/1‑90 (MQ=255) gtGCTCGTTC‑CCAAACCCA‑AAACCACCCAGGGCCGGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg < 2:440205/89‑1 (MQ=255) aTGCTC‑TTCCCCAAACCCAAAAA‑CCGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg < 1:347225/90‑1 (MQ=255) tctcAAACCCCA‑AAA‑CAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcct < 1:375692/90‑1 (MQ=255) aGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGcc > 1:221639/1‑90 (MQ=255) cGGAAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCa < 1:206814/90‑1 (MQ=255) | GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAA‑CAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCA > NZ_CP009273/2356073‑2356238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |