| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,769,914 | 0 | A | G | 57.1% | ‑1.1 / 10.1 | 15 | E57G (GAG→GGG) | yfjY | RadC family protein |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGC > NZ_CP009273/2769840‑2769996 | gcgcGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTat > 1:196136/1‑90 (MQ=255) aaCAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCt < 2:96496/90‑1 (MQ=255) ggCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCCGGGGGGGGGAGAATTTATGATGCTGTAt > 2:268544/1‑90 (MQ=255) ggCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCGGGGGGGGGGAGAAATTATTATTGTTTATTTGaa > 2:391853/1‑90 (MQ=255) ccACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGCGGGGAGAATTTTTGATGCTGTATCTGAAACAGCAGaaac > 2:300709/1‑88 (MQ=255) aCTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGGAGAATTTTTTATGGTGTATCTTAACCAGCAGAACCAg > 2:248529/1‑90 (MQ=255) ggCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGTGCGGGAAGAAATTTTGATGCTGTATCTGAAACAGCAGAACCCGTTTAt > 1:402919/1‑90 (MQ=255) tgctCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGGGGGAAGAATTTTTGATGCTGTATCTGAACCAGCAGAAACAGGTGAtttc > 2:9842/1‑88 (MQ=37) tCGTGACTGGCTGAAGCTGAAAATGGCGGGGGTGGGGCGGGGAGAATTTTTGATGCTGTATTTGAACCAGCAGAAACAGGTGATTGcccc > 2:14543/1‑89 (MQ=255) gACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGaa > 1:18012/1‑90 (MQ=255) ggCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGGAGAAATTTTTATGCTGTTTTTGAAACAGCAGAACCAGGTGGTTTGCCCCGAAAacc > 2:218946/1‑90 (MQ=255) ggCTGAAGCTGAAAATGGCGGGGCTGGGGGGGGAAGAATTTATGATGGTGTATCTGAACCAGCAGAACCAGTTGATTGGCCCCGAAAacc > 1:323259/1‑90 (MQ=255) gaaAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCgg < 2:199870/90‑1 (MQ=255) gggCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGc < 1:253418/90‑1 (MQ=255) gggCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGc < 1:308640/90‑1 (MQ=255) | GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGC > NZ_CP009273/2769840‑2769996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |