| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,775,223 | 0 | T | G | 52.9% | 16.5 / 19.9 | 17 | T234P (ACC→CCC) | cysE | serine O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGC > NZ_CP009273/3775136‑3775307 | tACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTAt < 2:306126/90‑1 (MQ=255) gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATgtgg > 2:391911/1‑90 (MQ=255) gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATgcgg > 1:428679/1‑90 (MQ=255) gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGGTgcgc > 1:253495/1‑89 (MQ=255) gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATgcgg > 1:343848/1‑90 (MQ=255) gCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCg < 2:428679/90‑1 (MQ=255) aTTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCCGGTGCAGCACCACg > 1:311819/1‑90 (MQ=255) tGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAAcc < 1:284344/90‑1 (MQ=255) tGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAAcc < 2:276620/90‑1 (MQ=255) gCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGGTGGGGCGGCCCCCGGTGCAGCGCCCCCGAAccag > 2:467888/1‑88 (MQ=255) gCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGCGGCGGCCCCCGGTGCAGCACCACGGAAcccg > 2:36639/1‑88 (MQ=255) tATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGGGGCACCGGGTGCAGCACCACGGGAACTgcgc > 2:83719/1‑90 (MQ=255) tATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGGGGGGGCGCCCGGTGGAGCACCACGGAAACCgcgc > 2:284344/1‑90 (MQ=255) gTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTcgcgc < 1:33089/90‑1 (MQ=255) aCAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCa < 1:83719/90‑1 (MQ=255) gCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAata > 1:383615/1‑90 (MQ=255) ccGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAatat < 2:57410/90‑1 (MQ=255) ggtgggATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGGTTTTcgc > 1:289983/1‑90 (MQ=255) | TACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGC > NZ_CP009273/3775136‑3775307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |