| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,224,171 | 0 | A | C | 53.8% | 4.2 / 14.9 | 13 | P162P (CCA→CCC) | pgi | glucose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base C (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGC > NZ_CP009273/4224083‑4224254 | cGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCAt < 2:379886/90‑1 (MQ=255) ttATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTcc < 1:411607/90‑1 (MQ=255) ttATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTcc < 1:425238/90‑1 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTGACCTCGGCCCCTACATGGGGGCCGAAGCTCTGCGGCCg > 1:192966/1‑90 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTTACCCCGGCCCCAACATAGGGACCGAAGATCTTCGGccc > 1:92978/1‑89 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTCTGACCTCCGCCCCAACATGGGGGCCGAAGCTCTGCGTCCg > 1:37502/1‑90 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTTACCTCGGCCCCTACATGGGGACCGAAGCTCTGCGTCCGTACaaaa > 1:373754/1‑90 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACaaaa < 2:363311/90‑1 (MQ=255) aaTCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCt < 2:43662/90‑1 (MQ=255) gACGTAGTGAACATCGGGATCGGCGGGTTTTACCCCCGCCCCTACAAGGGGACCCAAGCTCTTCGTCCGCGCAAAAAACACCCTAACAtt > 1:453301/1‑89 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTTTGACCTCCGCCCCTACATGGTGACCGAAACTCTGCGGCCGTACAAAAACCACCCGCACATGc > 1:37854/1‑90 (MQ=255) gTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCCCACAAAGTGGACGAAGCTCTTCGGCCGGGCAAAAAACACCCGAAAATACACctt > 2:289214/1‑90 (MQ=255) cGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATcgc > 1:264142/1‑90 (MQ=255) | CGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGC > NZ_CP009273/4224083‑4224254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |