| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 242,170 | T→C | G41G (GGA→GGG) | dpaA ← | peptidoglycan meso‑diaminopimelic acid protein amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 242,170 | 0 | T | C | 94.4% | 52.4 / NA | 18 | G41G (GGA→GGG) | dpaA | peptidoglycan meso‑diaminopimelic acid protein amidase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (6/11); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGG > NZ_CP009273/242084‑242254 | cTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGAcccc > 1:199195/1‑90 (MQ=255) cAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAActg < 1:159439/90‑1 (MQ=255) aGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAActgc < 2:115670/90‑1 (MQ=255) gTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAActgct < 2:409742/90‑1 (MQ=255) gTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGACGTAGACAGGGGACCCCATCAActgct < 2:133020/90‑1 (MQ=255) ttGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTtata < 1:256788/90‑1 (MQ=255) cTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATActc > 2:67323/1‑90 (MQ=255) cGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCtt < 2:199195/90‑1 (MQ=255) cccATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTg > 2:164744/1‑90 (MQ=255) tAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTg < 1:164744/90‑1 (MQ=255) agagATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTg > 1:9779/1‑89 (MQ=255) agagATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTg < 2:9779/89‑1 (MQ=255) gaTCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTag > 1:59661/1‑90 (MQ=255) cTTGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCaa < 2:59661/90‑1 (MQ=255) ttGAAGATCTGGATGTAGACAGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCaaa > 2:381566/1‑90 (MQ=255) gACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAAAGCTGCTGCTGGCCAGCAATCCGGCAAATGAAACGCa < 1:309647/90‑1 (MQ=255) aGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACgg < 1:69882/90‑1 (MQ=255) aGGGGACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACgg < 2:417059/90‑1 (MQ=255) ggggACCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACggg > 1:279350/1‑90 (MQ=255) | CTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGG > NZ_CP009273/242084‑242254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |