| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,613,576 | A→C | D67A (GAC→GCC) | marR → | multiple antibiotic resistance transcriptional regulator MarR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,613,576 | 0 | A | C | 89.5% | 52.6 / ‑0.6 | 19 | D67A (GAC→GCC) | marR | multiple antibiotic resistance transcriptional regulator MarR |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (9/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
CGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGC > NZ_CP009273/1613493‑1613658 | cgcgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTggg < 1:224321/90‑1 (MQ=255) gcgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGa > 1:333366/1‑90 (MQ=255) tgctctgctcTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGc < 1:158705/90‑1 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 1:241355/1‑90 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 2:130005/1‑90 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 2:375602/1‑90 (MQ=255) ctctgctctATCCGCTGAGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 2:190069/1‑90 (MQ=255) aTCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTg > 1:385462/1‑90 (MQ=255) ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGt < 1:219452/90‑1 (MQ=255) cGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTc < 2:385462/90‑1 (MQ=255) gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCt < 1:293622/90‑1 (MQ=255) agcggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTaa < 1:190069/89‑1 (MQ=255) cggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTagag > 2:459375/1‑90 (MQ=255) gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGc > 2:502/1‑90 (MQ=255) cgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCt < 2:180477/90‑1 (MQ=255) ccGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAgg > 2:361290/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGa < 2:115075/57‑1 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGa > 1:115075/1‑57 (MQ=255) tcggtcgCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc < 2:403546/90‑1 (MQ=255) | CGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGC > NZ_CP009273/1613493‑1613658 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |