Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273807,1770TG53.8% 7.7 / 13.0 13V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase BioC
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCA  >  NZ_CP009273/807097‑807240
                                                                                |                                                               
gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAAGTCCCGCGCAGGGGGCGGccct                                                        >  1:18560/1‑88 (MQ=255)
  gTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACCCGCAGGGGGCGGCCCTAg                                                      >  1:380781/1‑90 (MQ=255)
   tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGa                                                     >  2:40791/1‑90 (MQ=255)
                     ggACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGTGACGGCCTTAGATCTCTCGCCGCCAATGCt                                   >  2:199535/1‑90 (MQ=255)
                              ttGTGGACCTGGGTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGc                          <  1:106762/90‑1 (MQ=255)
                                      ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACg                                                             <  1:214023/47‑1 (MQ=255)
                                      ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACg                                                             >  2:214023/1‑47 (MQ=255)
                                      ctggctggATGAGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGACGGCCCTAGATCTCTCGCCGCCCAAGCTTTTTTAGGGACGCCAGa                  >  2:366455/1‑90 (MQ=255)
                                       tggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGaa                 >  2:100139/1‑90 (MQ=255)
                                                  gccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg      <  2:584726/90‑1 (MQ=255)
                                                  gccgccACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg      >  2:27164/1‑90 (MQ=255)
                                                  cccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg      <  1:405849/89‑1 (MQ=255)
                                                      ccACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCCCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCa  >  1:204283/1‑90 (MQ=255)
                                                                                |                                                               
GCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCA  >  NZ_CP009273/807097‑807240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑