Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG56.2% 10.0 / 17.4 16T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base G (9/0);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 8.74e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04
Rejected as consensus: Frequency below/above cutoff threshold.

GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822886‑823025
                                                                     |                                                                      
gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAg                                                    >  1:115157/1‑90 (MQ=255)
gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAg                                                    >  2:80885/1‑90 (MQ=255)
   tggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCg                                                 >  2:132972/1‑90 (MQ=255)
          cATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGAAGTCGGgtcgtc                                          >  2:387880/1‑90 (MQ=255)
                     cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa                               <  1:329883/90‑1 (MQ=255)
                        cTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGt                            <  2:282541/90‑1 (MQ=255)
                          ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                          <  1:387880/90‑1 (MQ=255)
                          ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                          <  1:475880/90‑1 (MQ=255)
                                aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCa                    <  2:163031/90‑1 (MQ=255)
                                   aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCCGTTCATgg                 >  2:88080/1‑89 (MQ=255)
                                     gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGGAt               >  2:354970/1‑90 (MQ=255)
                                        gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg            <  1:132972/90‑1 (MQ=255)
                                        gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGTCGGGTTGTCGAGAAAAAAAAAGTCCGGGTCATGCATCAg            >  1:176912/1‑90 (MQ=255)
                                             tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc       <  2:102511/90‑1 (MQ=255)
                                                  aGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAGCGAACAGgcc  >  1:317525/1‑90 (MQ=255)
                                                  aGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc  >  2:196583/1‑90 (MQ=255)
                                                                     |                                                                      
GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822886‑823025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑