| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,698,302 | 0 | A | C | 58.3% | 7.2 / 11.3 | 13 | G88G (GGT→GGG) | ydgJ | oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698213‑1698379 | agCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATC‑AACGACCACATGTTTa > 2:477737/1‑90 (MQ=255) gtttttcGCCCGCGCCCCCAGCCCCCGCGTTTGTGAGGGTGCCGGGGTAAGGGT‑TTTAAC‑AACGCCCACTTTTTTCCCCGCCTCAAgcgc < 1:540386/88‑1 (MQ=255) aGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCt > 2:191166/1‑90 (MQ=255) ctctCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATg > 2:424990/1‑90 (MQ=255) tgtgACGGTGGCAGGGTAAGGGT‑TTTAAC‑AACGGCCCCATGTTTGCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 1:537800/90‑1 (MQ=255) gtgACATTGCCA‑GGAAAAGGGTTTTAAC‑AACCCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTgg < 1:191166/90‑1 (MQ=255) aGTGCCACGGAAAAGTT‑TTTAAA‑AACGCCCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTa < 1:424990/90‑1 (MQ=255) cGGTAAAGGG‑TTTAACAAACCCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATg < 1:387741/90‑1 (MQ=255) aaGGT‑TTTATC‑AACGCCCAATTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATc < 2:313568/90‑1 (MQ=255) ttATC‑AACGCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCt < 2:544499/90‑1 (MQ=255) taacc‑AACGACCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCt < 1:477737/86‑1 (MQ=255) tATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTa > 2:101285/1‑90 (MQ=255) aCGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTg > 1:153708/1‑90 (MQ=255) gACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTGTCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg > 2:433760/1‑90 (MQ=255) | AGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698213‑1698379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |