| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,360,810 | 0 | A | G | 53.3% | 8.4 / 14.5 | 15 | G87G (GGA→GGG) | arnC | undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAA > NZ_CP009273/2360722‑2360877 | tGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGAt > 1:504964/1‑90 (MQ=255) ggTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGtt > 2:84705/1‑90 (MQ=255) aaGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTc < 2:460601/90‑1 (MQ=255) tCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTAGTCAcagt > 2:380917/1‑87 (MQ=255) gagaACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGTGCAACATTCAGCGATTATGGCGGGGTTTAGGCACGTTTCTGGCggc > 2:415646/1‑88 (MQ=255) atatTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAAttatta < 2:47909/90‑1 (MQ=255) gtgtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGTTTCTGGCGGCTTAATTATTTTCCtt > 2:72763/1‑90 (MQ=255) gtgtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAAGCCCGTTACTTGTGGCTTTATTTTTTACCtt > 2:561623/1‑90 (MQ=255) gtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCCCGTTACTGGGGGCTTTATTTTTTCCCtttt > 2:245659/1‑88 (MQ=255) aTTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGcc < 1:72763/90‑1 (MQ=255) ttttGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTTACGTTACTGGCGGCTTAATTTTTATCCCTGGTTCCg > 2:230730/1‑90 (MQ=255) tGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGAtc < 2:504964/90‑1 (MQ=255) cAACATTCAGCGATTATGGCGGGGGTTAGTCACGTTTCTGGGGACTTTATTATTTACCTTTTTGCCGGTCTCCCGAAACCCCCCgaaaaa > 1:241388/1‑90 (MQ=255) cAACATTCAGCGATTATGGCGGGATTTAGTCACGGTTCTTGCGACCTAATTTTTTTCCTTTTTGCCGGTCTCCCCAAACCCCCAgaaaaa > 1:279277/1‑90 (MQ=255) aaCATTCAGCGTTTAGGGGGGGATTCAGCCCCTTTCCGGGCGACTTAATTATTCCCCTTGATGCCGATCCCCAAAACCCGCCaaaaaaaa < 1:137053/90‑1 (MQ=255) | TGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAA > NZ_CP009273/2360722‑2360877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |