| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,020,300 | 0 | A | C | 54.5% | 3.0 / 11.0 | 11 | E391D (GAA→GAC) | guaD | guanine deaminase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGG > NZ_CP009273/3020216‑3020383 | gCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCAc > 2:40135/1‑90 (MQ=255) gcggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCa > 2:519734/1‑90 (MQ=255) gAGCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTc > 1:289290/1‑90 (MQ=255) gCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCg > 2:510549/1‑90 (MQ=255) gggCTTGGAGGATTTGTTTGGC‑ATCTTTTTCCCGGCCAAAGGGGGTGATTCGGGGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCt < 2:403215/90‑1 (MQ=255) gggCCTTGACGTTTTGTTTGCC‑ACTTTTTTCCCGGGCAAAGGGGCTTTTTTCGTGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCt < 1:206460/90‑1 (MQ=255) ggCCTTGGAGGTTTTGTTGGCAAATTTTTTCCCTGGAAAAGGGGCTGTTTTGGGGGTGATGAACCCCACCGCCACTCCGCTACAGCAGCt < 1:188768/90‑1 (MQ=255) ccTTGACGTTTTGATGGCC‑ACCTTTTTCCCGGGAAAAGAGGCTGTTTTGGGGGTGAGGAACCCCACCGCCACTCCGCTACAGCAGCTgcg < 1:467018/90‑1 (MQ=255) gCAAAGAGGTTTTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAAt < 1:430470/90‑1 (MQ=255) ggtgATGCACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGtt < 1:28397/90‑1 (MQ=255) gATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTggg > 1:68121/1‑90 (MQ=255) | GCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGC‑AACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGG > NZ_CP009273/3020216‑3020383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |