| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,531,801 | 0 | C | G | 57.1% | 15.4 / 27.6 | 21 | A353G (GCG→GGG) | yhgF | Tex family protein |
| Reads supporting (aligned to +/- strand): ref base C (3/6); new base G (12/0); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGA > NZ_CP009273/3531714‑3531885 | ccGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGcga < 2:62424/90‑1 (MQ=255) ggACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGcgacc < 2:516631/90‑1 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCCCCGGCAAACTGGTGGGGGCCGacac > 2:585404/1‑90 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGGGGTGCCCCCGGCAAACAGGGGGGGGCCCacac > 1:245146/1‑90 (MQ=255) gTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCCCTGGCAAACTGGTGGGGACCGACACCa > 2:520857/1‑90 (MQ=255) gTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCCCCGGCAAACTGGGGGGGACCGACACCa > 2:172707/1‑90 (MQ=255) tGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAt < 1:28296/90‑1 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGTGGGGGAAGCCACTGGCAAAATGGTGGGGGCCGACACCCTTTACCCCCCCACCGGGCAGGGCCCaaa > 1:531423/1‑90 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCCCTGGCAAAATGGTGGGGACCCACACCCTTTACCCCCACACCCGGCAGGCCGCcaa > 2:419102/1‑90 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCACTGGCAAACTGGTGGGGGCCGACACCATTTACCCGCACACCGGGCAGGCCGCcaa > 2:558403/1‑90 (MQ=255) aCTGGGGTAAAAGTGGCGGTGGGCGATGCCCCTGGCAAAATGGGGGGGGCCCACACCATTTTCCCGCACACCGGACAAGCCGCAAAaaca > 1:3745/1‑90 (MQ=255) tGGGGTAAAAGTGGCGGTGGTTGATGGCCCTGGCAAACTGGGGGGGGCCGACACCCTTTTCCCCCCCCCCGGGCAGGGCGGAAAagcggg > 2:345045/1‑87 (MQ=255) gTAAAAGTGGCGGGGGGCGGTGCCACTGGCAAACTGGTGGGGGCCCACAACATTTTCCCCCCCACCCGGCAGGCCGCAAAAACAGCGATg > 1:446741/1‑90 (MQ=255) aaaGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAcc < 2:77907/90‑1 (MQ=255) aaaGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAcc < 1:160443/90‑1 (MQ=255) aaaGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAcc < 1:585404/90‑1 (MQ=255) aGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGt < 1:172707/90‑1 (MQ=255) ggtCGGTGCCACTGGCAAACTGGTGGGGGCCGACACCATTTTCCCCCCCACCCGGCAGGCCGCAAAAACAGCGGTGACCGGTGGTGCGCt > 1:241868/1‑90 (MQ=255) gggcGATGCCACTGGCAAAATGGGGGGGACCGAAACCAATTTTCCGCACACCGGACCGGCCGCAAAAAGAGGGATGAACGCTGCTGCGCt > 1:274810/4‑90 (MQ=255) cGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtg < 2:241868/90‑1 (MQ=255) ggCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACAt > 1:313861/1‑90 (MQ=255) cAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATaa > 1:234450/1‑90 (MQ=255) ggtggCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGa > 1:282321/1‑90 (MQ=255) | CCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGA > NZ_CP009273/3531714‑3531885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |