| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,924,078 | 0 | T | G | 53.8% | 6.5 / 11.7 | 13 | T126P (ACC→CCC) | ravA | ATPase RavA |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGGCCGCCACCAGCAGACGCATCGGGATTTTTTCTACGTGTGCACCGTTGCGGAACTGGCGCTCGTTAATGGCGGTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGC > NZ_CP009273/3923993‑3924167 | gAGGCCGCCACCAGCAGACGCATCGGGATTTTTTCTACGTGTGCACCGTTGCGGAACTGGCGCTCGTTAATGGCGGTGAGCAAGGTATTa < 1:290546/90‑1 (MQ=255) ggCCGCCACCAGCAGACGCATCGGGATTTTTTCTACGTGTGCACCGTTGCGGAACTGGCGCTCGTTAATGGCGGGGAGCAAGGGATTAAg > 2:387587/1‑90 (MQ=255) gccACCAGCAGACGCATCGGGATTTTTTCTACGTGTGCACCGTTGCGGAACTGGTGCTCGTTAATGGCGGTGAGCAAGGTATTAAAAAtt > 2:354409/1‑90 (MQ=255) gCACCGTTGCGGAACTGGCGCTCGTTAATGGCGGGGGGGAAGGGATTAAGAATTGCCGGGCCCGCTTTTCAGATCTCATCCAGAAAGACa > 1:424805/1‑90 (MQ=255) ttGCGGAACTGGCGCTCGTTAATGGCGGGGGGGAAGGGATTAAAAATTGCCGGGGCCCCTTTTCAGATCTCATCCAAAAAGAAAAttttt > 1:315575/1‑88 (MQ=255) tGGCGCTCGTTAATGGCGGTGAGCAAGGGATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGc > 2:370993/1‑90 (MQ=255) tGGCGCTCGTTAATGGCGGTGAGCAAGGGATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAAATTTTGCTTCCGGc > 2:459954/1‑90 (MQ=255) tGGCGCTCGTTAATGGCGGTGAGCAAGGGATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAAACAATTTCTGCTTCCCGc > 2:348461/1‑90 (MQ=255) tCGTTAATGGCGGTGAGCAAGGGATTAAGAATTTCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTTTGCTTTCGGCAGGGaa > 2:259678/1‑90 (MQ=255) tAATGGCGGTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGc < 1:178006/90‑1 (MQ=255) gCGGTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGtt < 2:182997/90‑1 (MQ=255) ggTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTaa < 1:259678/90‑1 (MQ=255) ggTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTaa < 2:424805/90‑1 (MQ=255) tATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAgc > 1:306135/1‑90 (MQ=255) | GAGGCCGCCACCAGCAGACGCATCGGGATTTTTTCTACGTGTGCACCGTTGCGGAACTGGCGCTCGTTAATGGCGGTGAGCAAGGTATTAAGAATTGCCGGGCCCGCTTTCCAGATCTCATCCAGAAAGACAATTTCTGCTTCCGGCAGGTAACCGCTGGTTAAACGTTCATAGC > NZ_CP009273/3923993‑3924167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |