| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,964,452 | 0 | T | G | 55.0% | 16.6 / 21.6 | 20 | G320G (GGT→GGG) | wecB | UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (11/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCA > NZ_CP009273/3964363‑3964532 | ggcggcATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGt < 2:480409/90‑1 (MQ=255) cATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACggg > 2:347191/1‑89 (MQ=255) agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGTGCGGCTggg > 1:54069/1‑89 (MQ=255) agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGAAGGGGCGTCTggg > 2:134446/1‑89 (MQ=255) gaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTa < 1:134446/90‑1 (MQ=255) aaCCTGTGCTGGTGATGCGCGGTACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGTCTGGGAGGGACGGGTAAACAGCGaa > 2:224349/1‑90 (MQ=255) gcgATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGGACGGGTAAGCAGCGAAATTTCGGGGGAGGGAcgc > 2:288494/1‑90 (MQ=255) cgATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGAcgcg < 2:54069/90‑1 (MQ=255) gATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGt < 1:347191/90‑1 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGGACGGTGGGTCTGGGAGGCACGGATAAGCAGCGAATTGTCGAGGGAGTGACGCGGCTTTTtaaa > 2:105751/1‑90 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTTGTAGGCACGGATAAACAGCGAATTTTCGGGGAAGTGACGGGGCTTTTtaaa > 2:311863/1‑90 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGGAAGGTGCGTCTTGTAGGCACGGATAAGCAGGGGATTGGGGGGGGAGTGACGCGTCCTTTtaaa > 2:409053/1‑90 (MQ=255) gCGTCCGGAAGCGGTGACGGCGGGTACGGGGCGGCTGGTAGGGACGGGTAAGCAGCGAAATGTTGGGGGAGGGGAGCGGCTTTTtaaaaa > 1:126140/1‑88 (MQ=255) gTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGGACGGGTAAACAGCGGAATGTGGGGGGAGGGGGGCGGCTTTTtaaaaaag > 1:40064/1‑86 (MQ=255) gTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGGACGGGTAAACAGCGAATTGTCGGGGGAGGGAAGCGGCTTTTtaaaaaag > 2:546971/1‑86 (MQ=255) tCCGGAAGCGGTGACGGCGGGGGCGGTGGGGCTGGGAGGGACGGGTAAGAAGGGGAGGGTCGGGGGAGGGGCGGGGCTTTTTAAAGACGa > 1:260800/1‑90 (MQ=255) ggAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGaaaa < 1:551334/90‑1 (MQ=255) aaGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACg < 1:277580/90‑1 (MQ=255) aaGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACg < 2:76099/90‑1 (MQ=255) aGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGa < 1:564917/90‑1 (MQ=255) gACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCa < 2:60268/90‑1 (MQ=255) | GGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCA > NZ_CP009273/3964363‑3964532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |