| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,213,521 | 0 | T | G | 53.3% | 12.5 / 18.6 | 15 | K12N (AAA→AAC) | iclR | glyoxylate bypass operon transcriptional repressor IclR |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGT > NZ_CP009273/4213441‑4213604 | tCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCcg > 1:146173/1‑90 (MQ=255)tCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCcg > 2:174071/1‑90 (MQ=255) cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCcgc > 2:239881/1‑90 (MQ=255) aTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGCGGCAACGGCGGGGTTTTTTCCCCGtttt > 1:571750/1‑89 (MQ=255) tCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTcg < 1:239881/90‑1 (MQ=255) tCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTcg < 1:392890/90‑1 (MQ=255) aTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTg < 2:202781/90‑1 (MQ=255) aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCtt < 2:571750/90‑1 (MQ=255) gTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATGGGGGGGGCCCTGACAGTCTCTTTTTTTTTTTTCGTggga > 2:522605/1‑88 (MQ=255) tGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGGATGGGGGCGACCCTGACAGTCTCTTTTTTTTGTATCGTGGGAAtcat > 2:256887/1‑90 (MQ=255) tGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGGGGCCATGACAGGCTCTTTTTTTTTTTTTGTGGGAAtcat > 1:30582/1‑90 (MQ=255) gCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcattttc < 2:64034/90‑1 (MQ=255) gTGGCAACGGCGGGGTTTTTTCCCCGGTTCGCGGGGATGGGGGGGGCCATGACAGGCTCTTTTTTTTTTTTTGTGGGAAtcatttttttt > 1:394526/1‑86 (MQ=255) ggcggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt < 1:562755/90‑1 (MQ=255) ggcggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt < 1:64143/90‑1 (MQ=255) | TCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGT > NZ_CP009273/4213441‑4213604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |