| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 209,035 | 0 | T | G | 53.3% | 8.9 / 16.5 | 15 | V73G (GTG→GGG) | tilS | tRNA lysidine(34) synthetase TilS |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGCTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATT > NZ_CP009273/208949‑209119 | gcgcgcTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGcc > 1:437922/1‑90 (MQ=255)gcgcgcTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGcc > 2:568540/1‑90 (MQ=255) cATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTc < 1:479440/90‑1 (MQ=255) cATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGGGGTc > 2:504492/1‑90 (MQ=255) tgCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCAAAc < 1:568540/90‑1 (MQ=255) gCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGGGGGCGAAcg > 1:175612/1‑90 (MQ=255) aTCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAAcgcg > 2:170180/1‑90 (MQ=255) aTCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGTGGTCGAAcgcg > 1:400611/1‑90 (MQ=255) aTCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGTGGGCGGAagcg > 1:340973/1‑90 (MQ=255) tttAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACt < 2:400611/90‑1 (MQ=255) aCGCATTGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGTGGGCGAACGCGGACAACTTGCGCAAGAAGGGCTGGGCATTGAgggc > 1:162828/1‑88 (MQ=255) ttGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGgc > 2:105450/1‑90 (MQ=255) ttGCGAAAACGTCTGCCAACAGTGGCAGGGGCCGCTGGTGGGCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCCGgc > 1:232058/1‑90 (MQ=255) tCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCAc < 1:552749/90‑1 (MQ=255) gCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCAtt < 2:340973/90‑1 (MQ=255) | GCGCGCTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATT > NZ_CP009273/208949‑209119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |