| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,612,067 | 0 | A | G | 56.5% | 8.4 / 24.2 | 23 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base A (2/8); new base G (13/0); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.18e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCA > NZ_CP009273/4611979‑4612152 | gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCggggag < 1:193797/90‑1 (MQ=255) ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAAt < 1:543606/90‑1 (MQ=255) aCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTa > 2:465507/1‑90 (MQ=255) tctcTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGAGGGGAATTATCa > 2:306473/1‑90 (MQ=255) tctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGc < 2:104197/90‑1 (MQ=255) ctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGTGGGTGGGCGGGGGGGGGGATTATTAGCg > 1:462644/1‑90 (MQ=255) tGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGa < 1:498179/90‑1 (MQ=255) tGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGa < 1:401914/90‑1 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 2:459112/90‑1 (MQ=255) aagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTg < 1:360105/90‑1 (MQ=255) aCGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGGCGGGGGGGGGGATTTTTAGCGGTGGGTTAATGCACAAAACCCTg > 1:243745/1‑90 (MQ=255) cTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCACAACACCCTGCGGaa > 2:351856/1‑90 (MQ=255) cTGACCGTATTTAACGGTGAATATACTGGCGGGTGGTTGGTGGGGGGGGGGGATTATTAGCGAGGGAATTATGCACAACACCCCGCggga > 1:461902/1‑88 (MQ=255) tGACCGTATTTAACGGTGAATATACTGGCGGTTTGGTGGGCGGGGGGGGGGATTTTCAGCGATGGATTTATGCACAAAACCCCGCGGaaa > 1:373836/1‑90 (MQ=255) tGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGGaaa > 2:33508/1‑90 (MQ=255) tGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGgaa > 2:229047/1‑90 (MQ=255) gTATTTAACGGTGAATATACTGGCGGTTGGTTGGTGGGGGGGGGGGATTATCAGCGGTGGGTTATTGCGCAACACCCCGCGGGAATTCct > 1:292928/1‑90 (MQ=255) gTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCCGCGGGAATTCct > 1:76331/1‑90 (MQ=255) gTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCaaa > 1:91496/1‑90 (MQ=255) gTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAAAACCCTGCGGGAAATTCTCTGGATCaaa > 1:172425/1‑90 (MQ=255) aCTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCAcc < 2:461902/90‑1 (MQ=255) tGGCGGTTGGTTGGTCGGGGGGGGGAATTATTAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCa > 1:100594/1‑90 (MQ=255) tGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGGGAATTCCTCTGGGTCAAAAAATCAcccc > 1:137915/1‑89 (MQ=255) gttggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACa < 2:234266/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 1:312975/90‑1 (MQ=255) | GATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCA > NZ_CP009273/4611979‑4612152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |