Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9990TG57.7% 10.1 / 29.6 26V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/10);  new base G (15/0);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 2.07e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-05
Rejected as consensus: Frequency below/above cutoff threshold.

AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCA  >  NZ_CP009273/349911‑350083
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aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                     <  2:68246/90‑1 (MQ=255)
    ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAg                                                                                 >  2:556554/1‑90 (MQ=255)
      gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAt                                                                               >  2:29799/1‑90 (MQ=255)
         gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                            <  1:370465/90‑1 (MQ=255)
               cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGaaa                                                                      >  2:285183/1‑90 (MQ=255)
                    ggggACGCGCGAGATTAAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGAAAagggg                                                                 >  1:561307/1‑88 (MQ=255)
                                gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAgggggggggggg                                                     >  2:530670/1‑89 (MQ=255)
                                gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGCGCCGGAAGGGGAgggggggggggg                                                     >  1:573843/1‑89 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                              <  2:122269/90‑1 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTGTTAt                                            >  2:316676/1‑90 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGGGCCGAAAGGGGGGGGGGGGGGGGGGGTTGTcgt                                            >  1:540037/1‑88 (MQ=255)
                                              tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTTTCATTCCGa                                       >  1:111417/1‑90 (MQ=255)
                                               ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                      <  1:251642/90‑1 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGGAaggg                                  >  1:440723/1‑88 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGGCATTTCGAAagag                                  >  2:383530/1‑90 (MQ=255)
                                                   aGCCATCCGGGAGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTTCGAAagag                                  >  2:454526/1‑90 (MQ=255)
                                                       aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCTGAAAAGGCAGGGGGGGGGGGGGGTTTTCATTTCGAAagagaggg                              >  1:33586/1‑88 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGTCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                            <  2:449241/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                            <  1:110281/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                            <  2:133016/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                            <  1:530670/90‑1 (MQ=255)
                                                           gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                          <  2:571905/90‑1 (MQ=255)
                                                                            cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGCCTGGAAGATCGTGAtg         >  2:291300/1‑90 (MQ=255)
                                                                            cGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGAtg         >  1:59388/1‑90 (MQ=255)
                                                                                 gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg    <  2:450818/90‑1 (MQ=255)
                                                                                   tGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa  >  2:382679/1‑90 (MQ=255)
                                                                                        |                                                                                    
AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCA  >  NZ_CP009273/349911‑350083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑