| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,613,576 | A→C | D67A (GAC→GCC) | marR → | multiple antibiotic resistance transcriptional regulator MarR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,613,576 | 0 | A | C | 87.5% | 44.5 / ‑0.1 | 16 | D67A (GAC→GCC) | marR | multiple antibiotic resistance transcriptional regulator MarR |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (8/6); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
TTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGG > NZ_CP009273/1613489‑1613660 | ttACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGccc > 1:447702/1‑90 (MQ=255) aCCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTg < 2:447702/90‑1 (MQ=255) gcgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGa > 2:593267/1‑90 (MQ=255) gctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCt > 2:356704/1‑90 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg < 1:480568/90‑1 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTg > 2:295339/1‑90 (MQ=255) ctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGcc < 2:31345/90‑1 (MQ=255) cGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTc < 1:97799/90‑1 (MQ=255) cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGGATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTg > 2:311530/1‑90 (MQ=255) gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGc < 1:30303/90‑1 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGt > 1:88405/1‑70 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGt < 2:88405/70‑1 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGaaa > 1:559099/1‑90 (MQ=255) ccGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAgg > 2:533902/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt > 1:125842/1‑90 (MQ=255) aaCTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGa < 2:559099/90‑1 (MQ=255) ggtcgCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCgcgg > 1:413844/1‑90 (MQ=255) | TTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGG > NZ_CP009273/1613489‑1613660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |