| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 883,470 | 0 | T | G | 56.2% | 8.8 / 14.9 | 16 | intergenic (+55/+120) | rcdA/ybjL | DNA‑binding transcriptional regulator RcdA/aspartate:alanine antiporter |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGT > NZ_CP009273/883392‑883552 | aTGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTcc > 2:74257/1‑90 (MQ=255) agGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGGTGGGGTCCACAAAAAgg > 1:417288/1‑90 (MQ=255) ggTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATgggg < 2:93990/90‑1 (MQ=255) ttGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGGGGTGGGCCCCAAAAAAAAGGGAc > 1:315117/1‑90 (MQ=255) aGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGTGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTg > 2:264691/1‑90 (MQ=255) aGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTg < 1:359204/90‑1 (MQ=255) aGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGGCATCAAAGAAAAGCAGTg > 2:108148/1‑90 (MQ=255) aGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGGCATCAAAGAAAAGCAggg > 2:228088/1‑88 (MQ=255) aGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGGCATCAAAAAAAAGCAggg > 2:490288/1‑88 (MQ=255) tttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGGTCCACAAAATGGGGGCATCAAAGAAAAACAGTggga > 2:359204/1‑88 (MQ=255) ttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCAc < 1:430205/90‑1 (MQ=255) aaCAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTa < 2:458882/90‑1 (MQ=255) gAACCGAAATGGCGGGGGTGATGGGGTCCCCAAAAAGGGGACATCAAAAAAAAACAGGGGCACTAAATAAAACTGATGCCCCGCGGaaaa > 1:24411/1‑90 (MQ=255) gAACCGAAATGGCGGGGGTGATGGGCTCCCCAAAAAAGGGACATCAAAAAAAAACAAGGGGACTAATTAAGACTGATGGCCTGCGGgaaa > 1:353115/1‑90 (MQ=255) gAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGGCATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGGAAAGtttt > 1:130645/1‑88 (MQ=255) ggCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGt < 1:504380/90‑1 (MQ=255) | ATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGT > NZ_CP009273/883392‑883552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |