Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,175,9570AT64.3% ‑1.4 / 10.3 14I17F (ATT→TTT) zupTzinc transporter ZupT
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base T (9/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 5.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAT  >  NZ_CP009273/3175876‑3176036
                                                                                 |                                                                               
tGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGcgcg                                                                         <  2:17291/90‑1 (MQ=255)
                          gTTGTCGATGTCAGTCCCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAAcc                                               <  1:191752/90‑1 (MQ=255)
                          gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGGGCGGTTTTCGGCGTTCTCCGGCaaaaaac                                               >  2:508558/1‑88 (MQ=255)
                                           ctctCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGGGCGTTTTTTCGCCGTCTCGGGCAAAAAACCCCCAAACCCTTTCTgg                              >  2:540901/1‑90 (MQ=255)
                                            tctcATTCTGACCATACTGGCGGGGGGAGGCACCGTTTTTGGCGCGGTTTTCCGCGTTCTCGGGGGAAAACCCTCGAACCCCCTTCTGGc                             >  2:399975/1‑90 (MQ=255)
                                            tctcATTCTGACCATACTGGCGGGGGCAGGCACGTTTTTTGGAGGGTTTCTTGGCGTTTTCGGGGAAAAAACCCCGAAGCGCTTACttgg                             >  2:423151/1‑87 (MQ=255)
                                            tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCTCGGGCAAAAAACCCCGAAACGCTTTCTGGc                             >  2:175887/1‑90 (MQ=255)
                                                    tGACCATACTGGCGGGGGGAGCCACGTTTTTTTGCGCGTTTTTCGGCGGTCTCGGGCAAAAAACCCCCAACCGCTTTCTGGGGGTTTTGc                     >  1:196880/1‑90 (MQ=255)
                                                     gACCATACTGGCGGGGGGAGGCCCGTTTTTTTGGGCGCTTTTCGGCGTTCTCGGGGAAAAAACCCCCAACCGGCTACTCGCGgtttttct                    >  1:314179/1‑86 (MQ=255)
                                                     gACCATACTGGCGGGGGCAGCCCCGTTTTTTTGCGCGGTTCTCGGCGTTCTCGGGCAAAAAACCCCCAACCCCTTACTGGCGGTTTTGCt                    >  2:386359/1‑90 (MQ=255)
                                                          tACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCTCCGGCAAAAACCCCCGAACCGCTTACTGGCGTTTTTGCTAGGtt               >  2:152670/1‑90 (MQ=255)
                                                                  ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg       <  2:154597/90‑1 (MQ=255)
                                                                       aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt  <  1:400693/90‑1 (MQ=255)
                                                                       aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt  <  1:491288/90‑1 (MQ=255)
                                                                                 |                                                                               
TGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAT  >  NZ_CP009273/3175876‑3176036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: