| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,379 | 0 | C | G | 58.8% | ‑0.5 / 11.8 | 17 | G72G (GGC→GGG) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base C (4/3); new base G (5/5); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGC > NZ_CP009273/3738293‑3738462 | gCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGggcggc < 2:36926/90‑1 (MQ=255) caAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTg > 2:104926/1‑90 (MQ=255) aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAATCATGAATGCGGGGGGCTTGTc > 2:7566/1‑90 (MQ=255) gTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGc < 2:58140/90‑1 (MQ=255) gTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGc < 2:397615/90‑1 (MQ=255) gAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGGAAACATGAATGAGGGGGGGTTTTCAAAACGCCttgttg > 1:78237/1‑90 (MQ=255) gCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAAAGCATTGTTGAc > 2:69646/1‑90 (MQ=255) gCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGGAAACATGAATGCGGGGGGCTTGTCTAAACGCCttgttggc > 1:393894/1‑88 (MQ=255) cgggCGATTCCGTTCTTTGTGCGGGGGGGTAAAATCATAAATGGGGGGGGCTTGAAAAAACGCATTGTTGCCCTGCCGATGAAACtggtg < 1:246826/88‑1 (MQ=255) tgGCGATTCCTTTTTTTGTGCGGGGGGGAGAAATCATGAATGCGGGGGGTTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACtggtgg < 1:383821/90‑1 (MQ=255) ggCGATTCCGTTCTTTGTGCTGGCGGGGGGAATCATGAATGCGGGCGGGTTGTCCAAACGCATTGTTGACCTGCCGATGGAACTGGTggg > 1:68992/1‑90 (MQ=255) ggCGATTCCGTTCTTTGTGCTGGCGGGGGAAAACATGAATGGGGGCGGCTTGTCAAAAAGCATTGTTGAGCTGCCGGTGAAACTGGTggg > 2:246826/1‑90 (MQ=255) cTTTGTGCTGGGGGGTGAAATCATGAATGGGGGGGGCTTGCAAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAgg < 2:402510/90‑1 (MQ=255) ttGTGCTGGGGGGTAAAATCATGAATGCGGGGGGTTTGTAAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCg < 1:130545/90‑1 (MQ=255) tgCTGGGGGGTGAAATCATGAATGGGGGGGGTTTGTAAAAACGCTTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:104926/90‑1 (MQ=255) tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGc > 2:486861/1‑90 (MQ=255) tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGc > 1:307098/1‑90 (MQ=255) tGCGGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 2:166475/90‑1 (MQ=255) | GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGC > NZ_CP009273/3738293‑3738462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |