| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,538,749 | 0 | A | T | 57.1% | ‑1.5 / 14.2 | 14 | Y42F (TAT→TTT) | fimH | type 1 fimbria D‑mannose specific adhesin FimH |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base T (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAA > NZ_CP009273/4538660‑4538837 | gATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGGAGCGCCCATGTTTa > 1:159299/1‑90 (MQ=255) ggTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGGGGCGCCAAAGTTTTTTTAAAcc > 1:15296/1‑90 (MQ=255) aaTGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGccc < 2:449535/90‑1 (MQ=255) aTTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGGGGGAGCGCCCAATTTTTTGTAAAACTTTCGCCCCGCGTGAAagt > 1:409802/1‑90 (MQ=255) aTTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGGGGCCACCCCCATGTTTTTGTAAACCTTTCGCCCCTCGTGAAtgt > 2:420409/1‑90 (MQ=255) aCCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTTTGTAAACCTTGCGCCCGTCGGGAATGTGGGGCAAAACCTGGGCGTGGGTCtt > 2:262594/1‑90 (MQ=255) aTCCCTATTGGCGGTGGCAGCGCCCATGTTTTTTTAAAACTTTCGCCCCTCGTGAATGTGGGGGAAAACCTGGGCGTGGTTTTTTTGACg > 1:229526/1‑90 (MQ=255) aTCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAAACTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGGCGTGGGTCTTTTGACg > 2:328554/1‑90 (MQ=255) cccTATTGGCGGGGGCCCCGCCAATGTTTTTGTAAACCTTGCGCCCGTCGTGAATGTGGGGGAAAACCTGGTTGTGGGTCTTTCGgcgcc > 2:291734/1‑89 (MQ=255) gCGGTGGGAGCGCCAATGTTTTTGTAAACCTTGCGCCCCGCGCGGAAGTGGGGGAAAAACTGGTCGTGGGTCTTTCGGCGCCAAtttttt > 1:122938/1‑90 (MQ=255) gCGGTGGCAGCGCCAATGTTTTTTTAAAACCTGCGCCCGTCGTGGATGTGGGGGAAAAACCGGTCGTGGGTCTTTCGACGCCAATCtttt > 1:109680/1‑90 (MQ=255) cgcCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGa < 2:441830/90‑1 (MQ=255) tttATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCgg < 1:200662/90‑1 (MQ=255) ttATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGa > 2:543048/1‑90 (MQ=255) tATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGaa < 1:135944/90‑1 (MQ=255) | GATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAA > NZ_CP009273/4538660‑4538837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |