| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 262,051 | 0 | T | G | 58.3% | 6.3 / 11.5 | 12 | T72P (ACC→CCC) | BW25113_RS25425 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGG > NZ_CP009273/261969‑262124 | atcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTaaa < 2:132819/90‑1 (MQ=255) gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGTAAAAACt > 2:292709/1‑90 (MQ=25) tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGt < 1:332846/74‑1 (MQ=255) tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGt > 2:332846/1‑74 (MQ=255) tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAGGTAAAAACTGCAAg > 1:45617/1‑90 (MQ=25) cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATaa < 2:202428/90‑1 (MQ=255) gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAagt < 2:63617/90‑1 (MQ=255) gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGGAAAAACTGCCAAGGGGGGCCGGGTAAGAAGGCaaaccg > 1:87911/1‑86 (MQ=16) gtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCCGGTAAGGAGTCAACCTGTGAccc > 2:254651/1‑89 (MQ=21) aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGGCCGGGGAAGGAGTCAACCCGTGGCCcttctt > 1:2750/1‑90 (MQ=255) cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGACCGGGTAAATAGTCAAACTGTGACCCttcttcc > 2:337974/1‑89 (MQ=25) gCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCt < 2:441104/90‑1 (MQ=255) ccATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTg < 2:2750/90‑1 (MQ=255) tCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCGGATAAGTAGTCAACCTGTGACCATTCTTTGCTGACATCATCCctgtgg > 2:505652/1‑90 (MQ=18) | ATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGG > NZ_CP009273/261969‑262124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |