| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,613,576 | A→C | D67A (GAC→GCC) | marR → | multiple antibiotic resistance transcriptional regulator MarR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,613,576 | 0 | A | C | 96.3% | 88.9 / NA | 28 | D67A (GAC→GCC) | marR | multiple antibiotic resistance transcriptional regulator MarR |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (15/11); minor base G (1/0); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGC > NZ_CP009273/1613489‑1613661 | ttACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGccc > 1:60601/1‑90 (MQ=255)ttACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACGGAAAAAGGTATTGTCGGACGccc > 2:386566/1‑90 (MQ=255) gcgGCACGGTTTAAGGAGGTATGCTCTATCCGCCGCGCGGCGTGCATTACTCCGGTTGAACTGAAAAAGGTATTCTCGGTCGGCCTgggg > 1:352443/1‑89 (MQ=255) cgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAg < 2:157641/90‑1 (MQ=255) gTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGAc > 2:76803/1‑90 (MQ=255) tttAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGAcc < 1:86599/90‑1 (MQ=255) tAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCg < 2:216636/90‑1 (MQ=255) aGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTa > 1:70344/1‑90 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 2:238522/1‑90 (MQ=255) ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTg > 2:119957/1‑90 (MQ=255) ctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGcc < 1:119957/90‑1 (MQ=255) ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGt < 2:70344/90‑1 (MQ=255) gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCt < 1:125178/90‑1 (MQ=255) cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTg > 1:382799/1‑90 (MQ=255) gtgtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTg < 2:352443/90‑1 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCAc > 1:273548/1‑45 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCAc < 2:273548/45‑1 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGaaa > 2:125671/1‑90 (MQ=255) cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGaaa > 2:531175/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt > 2:169706/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt > 2:169730/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt > 1:523792/1‑90 (MQ=255) tGAACTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGcc > 2:177626/1‑90 (MQ=255) aaCTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTCGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGa < 2:198236/90‑1 (MQ=255) aCTGAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGaa > 1:136504/1‑90 (MQ=255) gAAAAAGGTATTGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAAccc > 1:355426/1‑90 (MQ=255) ttGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAg > 1:13122/1‑90 (MQ=255) tGTCGGTCGCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgc < 1:125671/90‑1 (MQ=255) ggtcgCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCgcgg < 2:382799/90‑1 (MQ=255) gtcgCCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCgcggc > 2:10344/1‑90 (MQ=255) | TTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGC > NZ_CP009273/1613489‑1613661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |