| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,785 | 0 | G | T | 54.5% | 11.4 / 17.1 | 11 | V433L (GTG→TTG) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCC > NZ_CP009273/333701‑333859 | ccGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTc < 2:292321/90‑1 (MQ=255) atTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGGTCGGGttt > 2:43093/1‑90 (MQ=255) ccGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGGTCGGGTTTGGGTCGCATGgaga > 2:164650/1‑90 (MQ=255) gcgcgcGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACg < 1:113285/90‑1 (MQ=255) ggCGGTGGTCGGGGTGGGTTTTTTTCTCGGGTTTGGATCGCATGAAGAACCGGGCGGCTCCCCCCTCGGGGCGATCAAAAaagcgaaaag > 2:271796/1‑88 (MQ=255) ggCGGTGATTGTGGTGGGTTTTTTGCTCGGGTTTGGATTGCATGAAGATCCCGGCGGCTCCCCCCACGAGACGATCAAAAaagcggaagc > 2:97596/1‑90 (MQ=255) ggCGGTGATCGTGATGGGTTTTTTGCTCGGATTTGGATCGCATGAAGAACCGGTCGGCCCCCCCCTCCAGACGATCAAAAaaacgaaaac > 2:153535/1‑88 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAAaagcgaaagc > 1:1260/1‑90 (MQ=255) gCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGaagcgaaagcg > 2:214170/1‑90 (MQ=255) aTCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATcgcc < 1:43093/90‑1 (MQ=255) aTCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATcgcc < 2:291568/90‑1 (MQ=255) | CCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCC > NZ_CP009273/333701‑333859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |