Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273684,9700AC58.3% 0.1 / 11.1 12G456G (GGT→GGG) lntapolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCGCGC  >  NZ_CP009273/684896‑685053
                                                                          |                                                                                   
gtgAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATc                                                                      >  2:175490/1‑90 (MQ=255)
               ggCGTCACGTTAGTGGTTAACCCCTCGCGGGTGACCTGCGGGATCATCGCCTGAATCCCCCCCTGCGGGCCAATCACCGCCGTAATGCCg                                                       <  1:41360/90‑1 (MQ=255)
                       gTTAGTGGTTAACCCCCCGGGGGTGAACTGGGGGATCACCGCCTGAATCCCCCCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGt                                               <  1:244184/90‑1 (MQ=255)
                       gTTAGTGGTTAACCCCCCGCGGGTGAACTGCGGGATCATCCCCTGAATCTCCCCCTGCGGGCCAATCTCCGCCGTAATGCCGTTGTTGGt                                               <  1:5836/90‑1 (MQ=255)
                          aGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGtgct                                            >  1:111645/1‑90 (MQ=255)
                                   ccccTCGCGGGTGTACCGGGGGCTTTCCGCCGGATTCCCCCCCGGGGGCCCCCCCACCGCCGTAATGCCGTTGTTCGTGCTGCGCAACAg                                   <  2:256195/88‑1 (MQ=255)
                                       tCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAgctgt                               >  1:291871/1‑86 (MQ=255)
                                        cgcgGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGgc                              >  2:14223/1‑90 (MQ=255)
                                                   tgggggATCATCGCCTGAATCCCCCCCGGGGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTcc                   <  1:200357/87‑1 (MQ=255)
                                                    gCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGGGGGCGCGCCAGCTCCa                  >  2:83706/1‑90 (MQ=255)
                                                          tccccGCCTGAATCCCCCCCGGGGGCCCAACCCCCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGcgcac            <  2:274368/86‑1 (MQ=255)
                                                                    aaTCCCCCCCTGCGGGCCAATCCCCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCgcgc  <  2:282976/90‑1 (MQ=255)
                                                                          |                                                                                   
GTGAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCGCGC  >  NZ_CP009273/684896‑685053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑