| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,791 | G→A | intergenic (+160/‑32) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,791 | 0 | G | A | 93.3% | 47.7 / ‑3.5 | 15 | intergenic (+160/‑32) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (10/4); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGG > NZ_CP009273/49706‑49871 | gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc > 2:297434/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAt > 1:227467/1‑90 (MQ=255) cagcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATc < 1:2544/90‑1 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa < 1:41413/90‑1 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:99756/90‑1 (MQ=255) tcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTg > 1:227050/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGa > 1:170807/1‑60 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGa < 2:170807/60‑1 (MQ=255) ccAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg < 2:52091/90‑1 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:280438/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 2:136006/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 2:49568/1‑90 (MQ=255) gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:22253/1‑90 (MQ=255) gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:228396/1‑90 (MQ=255) ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg > 2:363760/1‑90 (MQ=255) | GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGG > NZ_CP009273/49706‑49871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |