Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	765,675	0	A	C	57.1%	7.6 / 11.7	14	T748P (ACC→CCC)	mngB	mannosylglycerate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base C (0/8);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT  >  NZ_CP009273/765606‑765756                                                                      |                                                                                  aTTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCt                                                               <  2:112468/90‑1 (MQ=255) aTTAAAATGCCAGCCCCGGACTCACACCTACGTGGTCTGCTTTCTTGTCGCCTAATTTTATTGAGTTATCCCGGTACGCCAACCGCCGCt                                                               <  1:60131/90‑1 (MQ=255)                  ggACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGgc                                              >  1:172594/1‑90 (MQ=255)                       cacaACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAg                                         >  2:80981/1‑90 (MQ=255)                               ggtgtTCTGTTTTCTTGTCGCTTAATTTTTTTGATTTACCCCGGTCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  1:310903/89‑1 (MQ=255)                               cGTGGTCTTTTTTTTTGTCGCCTAATTTTATTGATTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  1:167116/90‑1 (MQ=255)                               cGTGGTCTTCTTTCTTGTCGCCTATGTTTTTTGAGTTATCCCGGTCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  1:354182/90‑1 (MQ=255)                               cGTGGTCTGTTTTTTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  2:74499/90‑1 (MQ=255)                               cGTGGTCTGCTTTCTTGCCGCCAAATTTTATTGAGTTATCCCGGTACGCAAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  2:172594/90‑1 (MQ=255)                               cGTGGTCTGCTTTCTGGTCGCCTAAGTTTATTGAGTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                 <  1:389365/90‑1 (MQ=255)                                   gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc                             >  2:146564/1‑90 (MQ=255)                                             ttGTAGCCTAAGTTTATTGCGTTATACCGGTACGCCAACCGCCGCTGGTGTAACTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAgtg                   >  1:371058/1‑90 (MQ=255)                                               gTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGtt                 >  1:307596/1‑90 (MQ=255)                                                              tGATTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAt  <  1:98973/90‑1 (MQ=255)                                                                      |                                                                                  ATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT  >  NZ_CP009273/765606‑765756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑