| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 52.9% | 8.0 / 20.7 | 17 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTA > NZ_CP009273/822898‑823050 | tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCga > 2:67818/1‑90 (MQ=255) acagtttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa < 2:181783/86‑1 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt < 2:66799/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:419947/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:70026/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCCGAAGGCGGGTCGTCGAGAAAAAGAAAGTGCGGGTCCTgt > 1:181783/1‑89 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGTCGTCGGGAAAAAGAAAGTCCGGGTCATGc > 2:419947/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc > 2:100837/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGTCGTCGAGAAAAAAAAAGTCCGGGTCATGCAt > 1:147786/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAAGGCCCGGAGTCGGGTGGTCCAGAAACAAAATGTCCGGGTCATGccc > 2:364252/1‑88 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:140411/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGGTATGCATCAGCGaaa > 1:68587/1‑89 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGGAGGGGGGTGGTCCAGAAAAAGAAAGTGCGGGTCATGCATCAGCGAAc > 1:327345/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGACGGTTCGTCGAGAAACAGAAAGTCCGGTTCATGCATCAGCGAACAgg > 2:199833/1‑90 (MQ=255) ggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCt < 2:9404/90‑1 (MQ=255) gggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCt > 2:313130/1‑90 (MQ=255) ggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTa < 2:68587/90‑1 (MQ=255) | TCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTA > NZ_CP009273/822898‑823050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |