Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,472,5680AG54.5% 3.0 / 10.2 11E29G (GAG→GGG) ynbBphosphatidate cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTC  >  NZ_CP009273/1472485‑1472643
                                                                                   |                                                                           
gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGGGGGaaa                                                                       >  1:135571/1‑90 (MQ=255)
gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTACCGGGGGGaaa                                                                       >  2:51629/1‑90 (MQ=255)
  tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGGGAAAAg                                                                     >  1:403210/1‑90 (MQ=255)
       aaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGt                                                                <  1:42885/90‑1 (MQ=255)
         aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGa                                                              <  1:278248/90‑1 (MQ=255)
                     acTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACg                                                  <  2:323295/90‑1 (MQ=255)
                              gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGAGGGAAAAGGGGGGGGGGGTAAATTACGTATTTGttc                                         >  2:255544/1‑88 (MQ=255)
                                tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTTACGGGGGGAAAAAGGGGGGGGGGTAAATTATGTATTTGTACCt                                       >  1:72359/1‑90 (MQ=255)
                                                   gATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAGGGGGGGGGGGTACATTAAGTATTTGTAACTGGGGGGGGATCGTCATTTg                    >  2:172575/1‑90 (MQ=255)
                                                         ccGTTTTCTGCTATGGCGGTTACCGGGGGGAAAAGGGGGGGGGGGTACATTACGTATTTGTTCCTGGGGGGGGATCGTCCTttttttttt              >  2:172145/1‑89 (MQ=255)
                                                                     aTGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTc  <  1:172145/90‑1 (MQ=255)
                                                                                   |                                                                           
GCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTC  >  NZ_CP009273/1472485‑1472643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑