| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,477 | 0 | G | A | 60.0% | ‑3.9 / 10.0 | 15 | E676K (GAA→AAA) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (0/9); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852394‑2852555 | gACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCg > 2:155750/1‑90 (MQ=255) tATCGATCGCATTTTTCCCCGCGTAGGCGCGGCAGATGCCCTGGCGCCCGGGCGCTCAACCTTTATGGGGGAGATGACTAAAACCGCCaa < 2:67360/90‑1 (MQ=255) gcgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCg > 2:349208/1‑90 (MQ=255) ggCGCGGCAGTTGCCCGGGCGCCCGGCCCCTAACCCTTTAGGGTGGAGAGGACGAAAACCGCCAATATTTTACATAACGCCACCGAATAc < 2:435924/90‑1 (MQ=255) cgGCAGATGCCCTGGCGCCGGGGCCCCCACCCTTTATGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTc < 1:155750/90‑1 (MQ=255) ggCAGACGCCCGGGCATCGGGCCCCCCAACCTTTCGGGGGGGGTGGCCTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 1:254537/90‑1 (MQ=255) cAGAGGCCCTGGCGCCGGGCCGCCCACCTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 2:237375/90‑1 (MQ=255) ggcccTGGCGTCGGGGCGCTCAACTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGtt < 2:204747/87‑1 (MQ=255) gggCCCCCACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 1:359075/90‑1 (MQ=255) cTCACCTTTTAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCg < 1:45717/90‑1 (MQ=255) cttttATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 2:90401/88‑1 (MQ=255) tgtgggggAGTTGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTc < 2:182455/88‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 2:377617/1‑90 (MQ=255) cTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAAc < 1:38791/73‑1 (MQ=255) cTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAAc > 2:38791/1‑73 (MQ=255) | GACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852394‑2852555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |