| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,357,755 | 0 | A | T | 53.3% | 1.0 / 14.5 | 15 | P530P (CCA→CCT) | yhcD | fimbrial biogenesis outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base T (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGTTTCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357667‑3357836 | cGGTTTCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCAt > 2:61013/1‑90 (MQ=255) aCTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCaa > 2:383716/1‑90 (MQ=255) tGGCAGCATCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAac < 2:361947/90‑1 (MQ=255) gcaTCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGc < 1:383716/90‑1 (MQ=255) caTCAACTTAGTCCTTCAGAAAATAATGAAGACGATGGTGATCTTTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAACCACCGcc < 1:431286/90‑1 (MQ=255) aTCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTTTTCTCCGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCca < 1:56019/90‑1 (MQ=255) tgtttCAAAAAATAATGAAGACGAGGGTGATTTTTTTACCGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGCCACACTGTATAc < 2:331801/87‑1 (MQ=255) tCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGc > 1:404543/1‑90 (MQ=255) tCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGc > 2:388090/1‑90 (MQ=255) taatGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGa > 2:429179/1‑90 (MQ=255) aGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATaa < 2:352528/90‑1 (MQ=255) aGACGAGGGTGATCTTTTCTACGCAAACATCAGTATTCCTTTTGGAAAACCAAACCCCGCCACACTGTATACTCGCCATGACGATCATaa < 1:77990/90‑1 (MQ=255) aTGGTGATCTTTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAAccc < 1:98766/90‑1 (MQ=255) tCATTATTCCTTTTGGCAGACAAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAg < 1:61013/90‑1 (MQ=255) attATTCCTTTTGCCAGACAAAACCCCGCCACCCTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc < 2:248041/88‑1 (MQ=255) | CGGTTTCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357667‑3357836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |