| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,744,897 | 0 | A | C | 53.8% | 7.5 / 13.4 | 13 | V111G (GTT→GGT) | yiaT | MipA/OmpV family protein |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTT‑CCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAG > NZ_CP009273/3744808‑3744986 | gCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTT‑CCCCAACGCTCGTGGTGATACCAGGCaa > 1:198110/1‑90 (MQ=255) caGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTT‑GCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATa > 2:399252/1‑90 (MQ=255) gTTGCTTTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCCCGGTC‑CCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGt < 2:429228/90‑1 (MQ=255) gTTACCCAGACCGTCCGCAGCTGCAGGGGTTTTCCCGGTC‑CCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATc < 2:249773/90‑1 (MQ=255) gAACGTCCGCAGCTGCAGAGGCTTTCACGGTT‑CCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTAt < 2:46221/90‑1 (MQ=255) cccGCGGCGGCAGGGGC‑TTCCCGGTTCCCCCACCGCTCGTGGTGATACCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGc < 1:121038/89‑1 (MQ=255) ccAGCTGCGGGGGCTTCCCCGTTC‑CCCCACCCCTGGGGGTGATCCCGGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGt < 1:247851/89‑1 (MQ=255) tGCAGAGGCTTTCACGGTC‑CCCCACCGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt < 2:422433/90‑1 (MQ=255) tGCAGAGGCTTCCACGGTC‑CCCCACCGCTCGTGGTGATCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt < 2:198110/90‑1 (MQ=255) tCACGTTT‑CCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt < 2:251834/90‑1 (MQ=255) aCGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAgg > 2:195723/1‑90 (MQ=255) tggtgATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAAt > 1:272996/1‑90 (MQ=255) tACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGc > 1:199560/1‑90 (MQ=255) tACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGc > 1:82541/1‑90 (MQ=255) aCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAg > 1:174915/1‑90 (MQ=255) | GCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTT‑CCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAG > NZ_CP009273/3744808‑3744986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |