| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 117,252 | 0 | T | G | 52.9% | 9.5 / 15.4 | 17 | T263P (ACC→CCC) | aroP | aromatic amino acid transporter AroP |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGTTGCTTT > NZ_CP009273/117168‑117333 | cGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCACg > 2:308448/1‑90 (MQ=255) cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGGCCCCGCATTAGGgaga > 2:213713/1‑90 (MQ=255) ttCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGGAAAGCGGGGCCACGGCATCAGGGagagg > 1:289886/1‑89 (MQ=255) gTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCACGGCATCAGTGAGAGCAGAACGGCTAAc > 2:382304/1‑90 (MQ=255) aTCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGGAAACCGGGGCCCCGGCATCAGTGGGAGGAGAACGGGTAAAGa > 1:377745/1‑90 (MQ=255) gTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCCCGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGaa > 2:264595/1‑90 (MQ=255) gTGGAAGATCAGCACAAACGGACTGGTATCGGCGGGAACGCGGGGGCACGGGGTCAGGGAGGGGAGAACGGCGTAAGCAACAATATAgga > 2:380802/1‑88 (MQ=255) cacaAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGGGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCg < 2:351635/90‑1 (MQ=255) cacaAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCg < 1:308448/90‑1 (MQ=255) aCTGGTATCGGCGGTAACCCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAAACAGGATGCGGTAGATAAc > 2:225123/1‑90 (MQ=255) ggTATCGGCGGTAAAGCGGGGCCACGGCATCAGTGAGAGCAGAAAGGCTAACGAAACAAAATAGAAAAACAGGAAGCGGGAGATAAACTg > 1:435828/1‑90 (MQ=255) ggcggTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGt < 2:71948/90‑1 (MQ=255) ggcggTAACGCGGGTCCACGGCATCAGGGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGGAGATAACCTGGGTAGt > 2:318942/1‑90 (MQ=255) gTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGTTGCt < 2:123194/90‑1 (MQ=255) gTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGTTGCt < 2:435484/90‑1 (MQ=255) gTAACGCGGGTCCACGGCATCAGGGAGAGCAGAACGGCTAACGAACCAATATAAAAAAACAAGAGGGGGTAGATAAACTGGTGATTTGCt > 2:229393/1‑90 (MQ=255) aaCGCGGGGCCACGGCATCAGTGAGAGCAGAAAGGCTAACGAACCAATATAGAAAAACAGGATGCGGGAGATAACCTGGGTAGTTGCttt > 1:159163/1‑90 (MQ=255) | CGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGTTGCTTT > NZ_CP009273/117168‑117333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |