| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 341,015 | 0 | G | A | 40.7% | 20.9 / 16.5 | 27 | intergenic (+313/‑100) | BW25113_RS01680/BW25113_RS01685 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (0/11); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTAC > NZ_CP009273/340930‑341098 | tgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGaaaa < 2:317533/90‑1 (MQ=255) gACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAat > 2:407930/1‑90 (MQ=255) aCATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAata < 1:370875/90‑1 (MQ=255) ttCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGc < 1:375374/90‑1 (MQ=255) tatGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTa < 2:66292/90‑1 (MQ=255) tatGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTa < 2:230427/90‑1 (MQ=255) atGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTaa < 2:44282/90‑1 (MQ=255) atGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTaa < 1:220338/90‑1 (MQ=255) gAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTATGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAgg > 2:332028/1‑90 (MQ=255) aaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTc < 2:398649/90‑1 (MQ=255) ttttttttttATCTTCCCCACCCCCCACAAGCCAAAAAAAAAATATGCTGAAAGCCTCAAATAAAAAACCGCCATTAGCTCATCAGGAag < 2:319477/88‑1 (MQ=255) ttttttCTTTATCTACCCCACCCCCCCCAACCCTAAAAAAAAATATGCTGAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAag < 1:318180/88‑1 (MQ=255) tCTGTATCTTACGCATCGCCCTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAg < 2:141420/90‑1 (MQ=255) ttACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc > 1:85393/1‑90 (MQ=255) tccGCACCCCACCCAAGCCGGAAAAAAAATATGCTGTAAGGCTCATACAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc < 2:226758/88‑1 (MQ=255) tACGCACCGCACTCAAGCCTAAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc < 1:74063/90‑1 (MQ=255) tACGCACCCCCCCCAGGCCTAAAAAAAAATTTGCTGAAGGCCTCATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc < 1:31349/90‑1 (MQ=255) tACGCACCCCCCCCAAGCCTAAAAAAAAATATGCTGTAAGGCTAATAAAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc < 1:158776/90‑1 (MQ=255) cATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGa > 2:185444/1‑90 (MQ=255) aTCGCACTCAAGCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt < 2:126948/90‑1 (MQ=255) aTCGCACCCAAGCCTAAAAAAAAATATGTTGTAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt < 2:391763/90‑1 (MQ=255) aTCCCACACAAGCCTGAAAAAAAATATGCTGAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt < 1:414067/90‑1 (MQ=255) tCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 1:213524/1‑90 (MQ=255) tCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 1:126948/1‑90 (MQ=255) gCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt > 2:368857/1‑90 (MQ=255) gCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt > 2:42595/1‑90 (MQ=255) cTGAAAAAAAATATGCTGAAAGGCTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTAc < 2:341886/90‑1 (MQ=255) | TGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTAC > NZ_CP009273/340930‑341098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |