| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,787,831 | 0 | G | C | 54.5% | 6.4 / 13.7 | 11 | D74H (GAT→CAT) | gabP | GABA permease |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base C (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCCTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTT > NZ_CP009273/2787757‑2787901 | tGGCCTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGGTGCCCCCCCCCATACCGGGTCGtttt > 2:99993/1‑90 (MQ=255) aTCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTTCCACCCCCCGTAACGGGTTGTTTTTCCCCt > 2:313510/1‑90 (MQ=255) ggtTATGATTATGCGGATGTTGGCGGAAATGGCGGGTGCCCCCCCCCATACCCGGTCGTTTTTCCCCCTTTCCGATAAAACCAATTGTCg > 1:156653/1‑90 (MQ=255) gATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTgggc < 1:184561/90‑1 (MQ=255) gATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTgggc < 1:41315/90‑1 (MQ=255) gATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTgggc < 2:268539/90‑1 (MQ=255) aTGCGGATGTTGGCGGAAATGGCGGTTTCCCCGCCCCCTACCGGTTTGTTTTCCACCTATGCCGATAAAAACCTTTGGCGCTgggcgggc > 2:252661/1‑90 (MQ=255) aTGCGGATGTTGGCGGAAATGGCGGGTGCCACGCCCCATACCCGTGCGTTTTTCTCCTCTGCCGGTAAAACCAGTGGGCGCGgggcgggc > 1:220758/1‑90 (MQ=255) tGGCGGAAATGGCGGTTGCCCCGCCCCCTACCGGTTCGTTTTTCCCCTATGCCGATAAATCCATTGGGCGCCGGGGGGGCTTTACCATCg > 1:286148/1‑90 (MQ=255) ggAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTg < 1:99993/90‑1 (MQ=255) aaTGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGtt < 1:252661/90‑1 (MQ=255) | TGGCCTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTT > NZ_CP009273/2787757‑2787901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |