Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,897,0510CA55.6% ‑0.2 / 12.7 9intergenic (+318/+1055)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (5/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATAT  >  NZ_CP009273/2896971‑2897126
                                                                                |                                                                           
aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTa                                                                    <  1:290537/90‑1 (MQ=255)
                            cTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACAATCATTAAACTCAAAAAAAAAAAAAACATCCAGAt                                        >  2:107703/1‑90 (MQ=255)
                              ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACCCATTAACCTCAAAAAAAAAAAAAAATTCCAGAttt                                      >  2:93323/1‑90 (MQ=255)
                                           cTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCaa                         <  1:202931/90‑1 (MQ=255)
                                           cTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCaa                         <  1:93323/90‑1 (MQ=255)
                                             aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACCCATTAACCCCAAAAAAAAAAAAAAAATCCACAATTTTTTCTTTAACaaaa                       >  2:182780/1‑88 (MQ=255)
                                                     aCAGCGTATAGAGGCGGTTTTGTAAAAACACTCCTTAACCTCAAAAAAAAAAAAAAATTTCAGATTTTTTTTTTAACCAATGaaaaaaaa               >  2:242371/1‑90 (MQ=255)
                                                       aGCGTATAGAGGCGGTTATTTAAAAACACTCCTTAGCCTCAAAAAAAAAAAAAAAATTCAGATTTTTTTTTTAAACAAAGaaaaaaaaaa             >  2:224943/1‑88 (MQ=255)
                                                                  gCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAatat  <  2:358825/90‑1 (MQ=255)
                                                                                |                                                                           
ATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATAT  >  NZ_CP009273/2896971‑2897126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑