| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,532 | 0 | T | G | 57.1% | 14.5 / 16.2 | 21 | *198G (TGA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (12/0); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTA > NZ_CP009273/4464443‑4464605 | cTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGt < 1:333796/90‑1 (MQ=255) aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCg < 1:70208/90‑1 (MQ=255) ggggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTTGGGGGGATGCGGGGCTTGAcc > 1:261981/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAacgc > 2:122263/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 1:84329/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAAGCGGGGAAgcgc > 2:231158/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTGGGAGGGATGCGGGCCTTTACCCGAAAGGCGGGGAAacgc > 2:183498/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 2:346504/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTTTTTAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 2:165394/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 2:114996/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTTAGGGGGGATGCGGGCCTTGACCCGAAAGGGGGGGAAgagc > 1:75130/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGAGATAAAAAAAATTTTTTAGGAATGAAGGGGGTGTTGACCCGAAAGGCGGGgaaaagc > 1:330577/1‑86 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTCTTTAGGAGGGGTGCGGGGCCTGACCCGAAAGGCGGGGAAACGCCt > 1:178445/1‑90 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGaa < 1:358339/83‑1 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGaa > 2:358339/1‑83 (MQ=255) tgtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGc < 2:261981/90‑1 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGcc > 2:51432/1‑90 (MQ=255) gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGGGGAGTGATGCGGCCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGtt < 2:330577/90‑1 (MQ=255) gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGtt < 2:295346/90‑1 (MQ=255) gCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAGGGCCTGCCGATAAGTTGTGATAAGa > 1:358445/1‑90 (MQ=255) gggAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGGTAAGTTGTGATAAGACaat > 2:95740/1‑90 (MQ=255) accTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa < 1:165394/88‑1 (MQ=255) | CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTA > NZ_CP009273/4464443‑4464605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |