| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 890,370 | 0 | C | G | 54.5% | 3.4 / 11.9 | 11 | intergenic (+18/‑77) | potF/potG | spermidine/putrescine ABC transporter substrate‑binding protein PotF/putrescine ABC transporter ATP‑binding subunit PotG |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base G (0/6); total (3/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCT > NZ_CP009273/890283‑890458 | gAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCgg < 1:250582/90‑1 (MQ=255) gCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGgc > 1:318870/1‑90 (MQ=255) gCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGgc > 2:83383/1‑90 (MQ=255) gcgcCCCCGCGCGGGGACAAAAGGGAAGAGCGAAAAAAAACCCGCAGTCGTAGATGCGGGGGGGGCGCCCCACCCCCGCCGGCAATTCGc < 1:342759/90‑1 (MQ=255) gcgcACCCGCGCGGGGACAAAAGTGAAGAGCGGAAAAAAACCCGCAGTCGTAGATGCGGGGGGGGCGCCCCACACCCGCCGGCAATTCGc < 1:79877/90‑1 (MQ=255) gcccccGGGGGTGGACAAAAGGGAAGAGCGAAAAATAATCCGCAGTCGTAGATGCGGGGGGGGCGCCCCACACCCGCCGGCAATTCGCAc < 1:143256/87‑1 (MQ=255) gcccccGCGCGGGGACAAAAGTGAAGAGCGAAAAATAATCCGCGGTCGTAGATGCGGGGGGGGCGCCCCACACCCGCCGGCAATTCGCAc < 1:137646/87‑1 (MQ=255) gcACCCGCGGGGGGACAAAAGTAAAGAGCGAAAAATAACCCGCAGTCGTAGATGCGGGGGGGGCGCACCACACCCGCCGGCAATTCGCAc < 1:83383/90‑1 (MQ=255) gcgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATgg > 1:234448/1‑90 (MQ=255) gTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCCCACCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcaca < 2:109352/90‑1 (MQ=255) gagCGAAAAATAACCCGCAGTCGTAGATGCGGGGGGGGCGCACCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTc < 2:318870/90‑1 (MQ=255) ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCt > 2:322205/1‑90 (MQ=255) | GAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCT > NZ_CP009273/890283‑890458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |