| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,163,022 | Δ1 bp | coding (830/900 nt) | yegS → | lipid kinase YegS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,163,019 | 0 | A | . | 92.9% | 45.2 / ‑1.3 | 14 | coding (827/900 nt) | yegS | lipid kinase YegS |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (5/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
ATATTATCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAA > NZ_CP009273/2162932‑2163096 | atatTATCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCaaa > 2:56161/1‑90 (MQ=255) atCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAttttc > 1:336010/1‑89 (MQ=255) aaGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCatat < 1:560596/90‑1 (MQ=255) gcgcTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGa > 2:537692/1‑90 (MQ=255) gcTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGaaa > 2:253167/1‑90 (MQ=255) ggTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGc < 1:379916/90‑1 (MQ=255) ttCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAgcgttgcg > 2:54903/1‑90 (MQ=255) gCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTc < 1:235524/90‑1 (MQ=255) cacaCGACATCACCTTTAATCTTGATGGTGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGAtt < 1:291507/90‑1 (MQ=255) cacaCGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGAtt < 1:20100/90‑1 (MQ=255) cACCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGAt < 1:56141/90‑1 (MQ=255) aCCTTTAATCTTGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGAtt > 2:198738/1‑90 (MQ=255) tGATGGCGAACCGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTg > 2:539062/1‑90 (MQ=255) ccGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCaa < 1:133629/90‑1 (MQ=255) ccGTTGAGTGGGC‑AAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCaa < 1:54903/90‑1 (MQ=255) | ATATTATCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAA > NZ_CP009273/2162932‑2163096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |