Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,060,988	(A)6→5	intergenic (+240/‑53)	yccE → / → agp	YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,060,983	0	A	.	100.0%	43.0 / NA	11	intergenic (+235/‑58)	yccE/agp	YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (3/8);  total (3/8)

GCATAGTTTGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGG  >  NZ_CP009273/1060895‑1061032                                                                                         |                                                  gCATAGTTTGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGGaa                                                  >  2:484681/1‑90 (MQ=255)    tAGTTTGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAACCATTTTGCGAACCTGTTCCCGGaaaaa                                               <  2:39530/90‑1 (MQ=255)        ttGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGGaaaaagtca                                           >  1:399728/1‑86 (MQ=255)         tGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCat                                         >  1:272658/1‑90 (MQ=255)          gCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCata                                        >  2:249320/1‑90 (MQ=255)            gTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATAtt                                      <  2:104876/90‑1 (MQ=255)                   cTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTca                               <  1:484681/90‑1 (MQ=255)                      gCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCacac                            <  1:152915/90‑1 (MQ=255)                      gCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCacac                            <  1:249320/90‑1 (MQ=255)                      gCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCacac                            <  1:269551/90‑1 (MQ=255)                      gCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCacac                            <  2:268218/90‑1 (MQ=255)                      gCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCacac                            <  2:483133/90‑1 (MQ=255)                                              aaaaaCAGGCGTTCAACAACCCATTTTGCGAACCTGTTCCCGGaaaa                                                <  1:84488/47‑1 (MQ=255)                                              aaaaaCAGGCGTTCAACAACCCATTTTGCGAACCTGTTCCCGGaaaa                                                >  2:84488/1‑47 (MQ=255)                                               aaaaCAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAagag   <  2:561215/90‑1 (MQ=255)                                                aaaCAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGG‑AAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAgg  >  2:409044/1‑90 (MQ=255)                                                                                         |                                                  GCATAGTTTGCGTCAAACCTTGCCTGTTTTTGAAGATGTATATAGAAAAACAGGCGTTCAACAAGCCATTTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGG  >  NZ_CP009273/1060895‑1061032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑