Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,240,670	Δ1 bp	coding (55/441 nt)	BW25113_RS06225 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,240,667	0	G	.	100.0%	29.0 / NA	8	coding (52/441 nt)	BW25113_RS06225	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (4/4);  total (4/4)

ATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGC  >  NZ_CP009273/1240582‑1240741                                                                                       |                                                                           aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAATgggcg                                                                        <  1:217836/90‑3 (MQ=255)                            ttttGGTGTGATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTgggcct                                            >  1:313643/1‑88 (MQ=255)                                  tgtgATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGaa                                      <  1:119039/90‑1 (MQ=255)                                  tgtgATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGaa                                      <  1:357175/90‑1 (MQ=255)                                       ttCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACAttt                                 <  2:411540/90‑1 (MQ=255)                                                      taagaGACAGGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATc                   <  2:428234/86‑1 (MQ=255)                                                                gTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGCTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAg         >  1:312923/1‑90 (MQ=255)                                                                     cAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTaa    >  2:179867/1‑90 (MQ=255)                                                                       gTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGc  >  2:106896/1‑90 (MQ=255)                                                                                       |                                                                           ATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACA‑GTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGC  >  NZ_CP009273/1240582‑1240741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑