| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,417,788 | (C)6→7 | coding (69/120 nt) | BW25113_RS26130 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,417,782 | 1 | . | C | 92.3% | 29.7 / ‑2.3 | 13 | coding (75/120 nt) | BW25113_RS26130 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (5/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA > NZ_CP009273/1417693‑1417862 | ttGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCt < 1:4680/90‑1 (MQ=33) ggTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGac < 2:226357/90‑1 (MQ=255) gTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGaca < 1:186956/90‑1 (MQ=255) tGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACAcc < 2:500103/90‑1 (MQ=255) cacaTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGc < 1:305052/90‑1 (MQ=11) cTGTCAGTGAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGattatt > 2:530794/1‑90 (MQ=11) cagtGCGTGAAGCCACCACCGCCCCCGGCGTGGATAATGCAGCCCC‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTc < 1:71237/90‑1 (MQ=11) gtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCg > 2:490539/1‑90 (MQ=255) gCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCggg > 2:151441/1‑90 (MQ=255) ccaccaCCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCa < 1:497779/48‑1 (MQ=11) ccaccaCCGCCTCCGGCGTGGATAATGCAG‑CCTCCCCCCCGACTGGCa > 2:497779/1‑48 (MQ=14) cGTGGATAATGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc < 1:478835/90‑1 (MQ=255) aTGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa > 1:433563/1‑90 (MQ=255) aTGCAG‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa < 1:530794/90‑1 (MQ=255) | TTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA > NZ_CP009273/1417693‑1417862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |