| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 53.8% | 0.6 / 15.3 | 13 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base T (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGC > NZ_CP009273/1213778‑1213945 | cGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGtt > 1:13050/1‑90 (MQ=255) ttGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGAtt > 1:410928/1‑90 (MQ=255) atttttGGCGCTTCGCTTTTTTTTTCGCCGTCTTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTACCCGCCGCGATTTAAACCtt < 2:445745/88‑1 (MQ=255) atttttAGCGCTTCGCTTTTTTTTCCCCCGCCTTTTGCCGAGGATTTTTTAAATTCCCGTGAAATTTTTCCCCGCCGCGATTTAAACCtt < 1:481026/88‑1 (MQ=255) ttAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTc > 2:48649/1‑90 (MQ=255) atatTTTTCCGCCGATTTTTGTCGACGAGTTTTAAAATTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCg < 1:48649/90‑1 (MQ=255) tttCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTg > 2:478759/1‑90 (MQ=255) cgccgcCGTTTGCCGAGGAGTTTTAAATTTTCCGTGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:518209/90‑1 (MQ=255) cGCCGACGTTTGCCGACGAGTTTTAAAATTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:417426/90‑1 (MQ=255) cgaGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTtgatga > 1:39019/1‑90 (MQ=255) tCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:542949/90‑1 (MQ=255) tCCGTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:410928/90‑1 (MQ=255) cccGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 1:478759/89‑1 (MQ=255) | CGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGC > NZ_CP009273/1213778‑1213945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |