| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,991 | 0 | A | C | 54.5% | 8.0 / 12.2 | 11 | G76G (GGT→GGG) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGC > NZ_CP009273/1377903‑1378078 | gagtgtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGGGAAGGCATGCAAAACTTCATAGAAAAACGGATTAc > 1:336605/4‑90 (MQ=255) atCACGTCGAACCCTTCTTCTTCAGCTTTGGCGGTGATGGCATGAAAAACTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGaa < 2:259788/90‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:87341/1‑90 (MQ=255) ctttcttcttcATTTTGGGGGGTGATGGCAGGAAAAACTTCAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 1:472303/88‑1 (MQ=255) ccTTCTTCTTCAGCTTTGGGGGTGATGGCATGAAAAACTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 2:184737/90‑1 (MQ=255) ggggTGATGGCAAGAAAAACTCAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa < 1:482847/88‑1 (MQ=255) tGGCATCAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAag < 1:560040/90‑1 (MQ=255) tCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAg > 1:586036/1‑83 (MQ=255) tCAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAg < 2:586036/83‑1 (MQ=255) aaaaaaaaaCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGc < 2:496867/86‑1 (MQ=255) ttACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGc > 1:553586/1‑90 (MQ=255) | GATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGC > NZ_CP009273/1377903‑1378078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |